Southern blotting. A researcher transfers an electrophoresis gel containing DNA into a tray of salt solution prior to blotting the DNA onto nitrocellulose paper. The purple dye shows how far electrophoresis has progressed. Southern blotting is a technique to reveal specific fragments of DNA (deoxyribonucleic acid) in a complex mixture. The total DNA is cut into fragments and separated according to size by gel electrophoresis. The DNA is then blotted onto a sheet of nitrocellulose placed on top,and a radioactive DNA probe added to the nitrocellulose. The resulting banding pattern,revealed by autoradiography,can be used to map the structure of particular genes | |
Lizenzart: | Lizenzpflichtig |
Credit: | Science Photo Library / King-Holmes, James |
Bildgröße: | 4724 px × 3743 px |
Modell-Rechte: | nicht erforderlich |
Eigentums-Rechte: | nicht erforderlich |
Restrictions: | - |