CRISPR-CAS9 gene editing complex. Computer illustration showing the molecular structure of the CRISPR-associated protein Cas9 in complex with guide RNA (ribonucleic acid,red) and target DNA (deoxyribonucleic acid,orange-fuchsia) containing a canonical 5'-NGG-3' PAM (yellow). Cas9 is a nuclease protein associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. It uses a guide RNA sequence to cut DNA at a complementary site. Seen here: Alpha-helical lobe (grey),HNH domain (blue),RuvC domain (pale blue),C-terminal-domain (pink),topo homology domain (pale pink),arginine-rich helix (light fuchsia),guideRNA (red),target DNA (orange),non-target DNA (fuchsia) |