Human genome research: computer analysis of DNA sequencing autoradiograms,used to decode the base sequence,or chemical blueprint,of lengths of DNA. The two strands of nucleotides which form DNA are joined by base pairing; adenine (A) always pairs with thymine (T),guanine (G) with cytosine (C). Hence the progression of its bases defines a section of DNA. Decoding a sequence is done by electrophoresis through an agarose gel,where fragments of radioactively-tagged DNA separate according to size. The banding pattern is revealed under ultraviolet light. A permanent copy obtained by blotting with radiographic film,serves as input for the computer analysis | |
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Credit: | Science Photo Library / Plailly, Philippe |
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